British Apogee insight into the subtle flow cytometry technology innovation and application
Puyang Linshi Medical Supplies Co., Ltd. , https://www.linshimedical.com
January 06 11:07:40, 2023
British Apogee insight into the subtle flow cytometer
- unique time stamp function
In recent years, small and medium-sized personal flow cytometers have become more and more popular among users because of their low price, easy operation and low after-sales maintenance cost. However, since flow is originally used in cell biology-related research, traditional instruments are designed for mammalian cells, and small particles such as bacteria, viruses, nanoparticles, and platelets cannot be reached most. Good detection results.
In response to this situation, the British Apogee company broke through the tradition and launched the A50-Micro series of flow cytometers, its ultra-high scattered light sensitivity (100nm) and resolution (10nm), enabling it to perform high-quality routine cell analysis. At the same time, it has an unrivaled advantage in the field of microparticle detection. Superior performance, low cost and maintenance, easy to operate, and compact, Apogee greatly expands the application of flow cytometry, becoming the only flow cytometer that can help you "see the subtle". Due to its stable performance and excellent sensitivity, Apogee has become the US military-sponsored brand for the rapid detection of bacteria and diseases such as anthrax spores and plague.
Technological innovation
Highlight 1: Nano-level resolution and sensitivity
The A-50 Micro uses a photo-coupled quartz cup flow cell with a numerical aperture of 1.2 or greater. In addition, the scattered light uses multi-angle light scattering (MALS) technology, which can set different lasers for scattered light detection (violet light has higher scattering light resolution than blue light), reaching an amazing nanometer level. The resolution can reach 10nm and the scattered light sensitivity reaches 100nm.
Highlight 2: The only flow cytometer with a wide sample detection concentration ( 10 2 ~ 10 9 cell / ml )
For traditional flow patterns, sample concentration is a very important sample preparation indicator. Too high or too low sample concentration will affect the accuracy of the test results. Therefore, such samples need to be diluted or concentrated before loading, but this causes operational complexity on the one hand and human error on the other hand. The use of Apogee breaks the limits of sample concentration detection and greatly expands the diversity of streaming applications.
Highlight three: 100,000 / sec superior detection speed
Detection speed is one of the most important performances of flow cytometry. Under the precursors that ensure accurate detection results, the faster the experiment, the less time the experiment consumes. Especially for some experimental samples that need to analyze a large amount of data, such as Minimal residual disease (MRD), detection of mutant cells, etc., it is often necessary to collect millions or even millions of cells for analysis. The detection speed of Apogee is Ten times the regular flow, you can save more than 90% of the time.
Highlight 4: Ultra-precise sample control with a minimum flow rate of 0.7 ul/min
The loading speed is very critical to the accuracy of the results. Although too fast speed will save time, it will lead to an increase in the error CV. For some experiments with stricter control of CV values, such as cell cycle analysis, etc., Reduce the sample loading speed. Apogee uses a high-precision stainless steel syringe pump that controls flow rates to an astonishing 0.7 ul/min, breaking the limits of traditional flow and enabling customers to obtain more accurate data.
Highlight 5: Timestamp " timestamp " function
The time stamp function is widely used in many industries. Apogee first applies this function to the data acquisition of flow cytometry, which ensures the authenticity and integrity of the data acquired by the instrument, and can determine the instrument according to the form of the data. Performance and quality of the sample.
Application field
including but not limited to
Ø Microbiology - can distinguish overlapping groups in traditional flow detection by scattered light
Ø Genetics - Archaea, cell cycle research
Ø Virology - small volume detection
Ø Immunology - lymphocyte phenotypic analysis, CD4 absolute count
Ø Conventional life science research—microsomal and exosome, platelet, and nanoparticle detection;
Apoptosis/viability, mitochondrial membrane potential analysis, plant ploidy analysis, DNA content analysis, and the like.
Ø Food and beverage process development and testing
Ø Wastewater plant monitoring
Ø Rapid assessment of water quality
Puri McGrady invites you to experience Apogee – the best cytometer for sensitivity and resolution!