What is the principle of dialysis? How long does it usually take to complete?

What is the principle of dialysis? How long does it usually take to complete?

Dialysis means that the solute is selectively diffused through the semi-permeable membrane driven by the concentration gradient to achieve large/small molecular separation, and the concentration on both sides of the membrane is balanced, that is, the solute of the small particles passes through the membrane, while the macromolecular substance cannot pass and is trapped. On the other side of the film.

If the sample is placed in the dialysis membrane tube, the small particle material can be removed by replacing the dialysis buffer outside the membrane tube, thereby achieving the purpose of purifying the macromolecular substance.

In general, 3-5 times of buffer is replaced within one day, and then dialysis is performed overnight at room temperature on a mixing table to achieve efficient dialysis results. Standard dialysis operations typically take 16-24 hours.

Many factors affect dialysis efficiency, including: diffusion coefficient, pH, temperature, time, substance concentration, sample volume, dialysis buffer volume, number of dialysis buffer replacements, membrane surface area, membrane thickness, molecular charge, and buffer agitation. rate.

The picture below is a simple illustration of dialysis:

More laboratory dialysis questions are welcome to consult Toscience!

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