In the Western experiment, the protein Marker can monitor the migration of proteins in SDS-PAGE, monitor the membrane transfer efficiency, and determine the molecular weight of the protein. Choosing the right protein Marker is one of the prerequisites for the success of western blot experiments. There are two types of protein Marker: one is unpre-marked Marker, which is a broad molecular weight protein standard, a high molecular weight protein standard and a low molecular weight protein standard; the other is a pre-dyed Marker, that is, a single color pre-dyed and multi-color pre- dye.

The unpre-stained protein Marker is the simplest and most accurate one. Since there is no dye molecule attached or the size of the label is exactly the size of the protein, it is necessary to accurately determine the size of the protein. Most of Marker now uses pre-mixed markers to facilitate comparison of proteins of different sizes.

The pre-stained protein Marker is a mixture of well-purified proteins that can be directly observed during electrophoresis or during membrane transfer by covalent coupling with the dye. The presence of the pre-stained Marker facilitates our experiments. This protein molecular weight standard helps us detect electrophoresis and estimate mobility during electrophoresis, after electrophoresis, and after transfection. It is worth noting that the pre-stained protein Marker is covalently coupled with the dye, so there may be some changes in the migration characteristics when electrophoresis under different buffer conditions, which may lead to some deviations, so it is not suitable for precise localization of proteins.

Lianke Bio-off-the-shelf ready-to-use pre-stained protein Marker can be loaded directly without boiling, and the strips are sharp, the color is stable, and the transfer efficiency is high.