Stability Analysis of Chlorophyll Meter for Toona sinensis
The growth of citron and the determination of chlorophyll content in plant leaves are an important test. In practical applications, chlorophyll is a plant physiological and biochemical study and post-harvest chlorophyll analyzer measurement parameters are also stored and processed. It is also an important indicator to measure quality. Dehydrated vegetables. Foods that extract pure natural chlorophyll from fruits and vegetables are natural colorants and can be widely used in food, cosmetics and other industries. Pick ripe, unaged, fresh red citrons, rinse with water, and drain. After chopping, a small amount of calcium carbonate and a small amount of cellulase are added to grind to neutralize the cell liquid, thereby protecting chlorophyll and increasing the leaching rate. It was filtered through a Buchner funnel, and the filtrate was concentrated by evaporation in a water bath at 90 ° C to 100 ° C. The chlorophyll extraction, filtration, concentration and other processes should be carried out in a dark environment to prevent chlorophyll photolysis, and the content can be determined by a chlorophyll meter. The optimum extraction conditions for the chlorophyll of the red citron were acetone-anhydrous ethanol mixed extractant, leaching for 20 h, ratio of material to liquid of 1:8, and temperature of 60 °C. The chlorophyll analyzer analysis showed that the primary and secondary relationships affecting the extraction factors of chlorophyll were the ratio of material to liquid ratio > extraction temperature > extraction time. The chlorophyll of red camphor is stable in the range of less than 100 °C, and the stability is better at pH 6~10, but it is easy to photolysis; it is stable to glucose, but unstable to citric acid; the chlorophyll content of red camphor is rich, thermal stability Very high, the extraction method established by the chlorophyll tester is simple and easy to implement, and the market prospect is broad. There was no significant change in color after adding 0.1 g of glucose and 0.1 g of citric acid to the chlorophyll extract of red camphor. The results of chlorophyll tester at 663nm and 645nm showed that the proper amount of glucose had no significant effect on the stability of chlorophyll. The effect of citric acid on its stability was significant, which may change the acidity of the extract with citric acid. Conditional.
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