Introduction:

This study demonstrates how the CatchPoint® cAMP Fluorescence Kit can be used to monitor the effects of adenylate cyclase activator forskolin on HEK293 cells in a SpectraMax® i3 multi-plate reader (Figure 1). G protein-coupled receptors (GPCRs) are important transmembrane proteins that direct extracellular signals into the cell, causing cascades of signal amplification, leading to changes in the activity or expression of certain proteins in the cell [1] . 3', 5'-phosphorylated cyclic adenosine (cAMP) acts as a second messenger involved in downstream reactions following GPCR activation. When an extracellular ligand acts on a GPCR, the conformation of the GPCR changes, activating the intracellularly linked G protein. The next signaling pathway is related to the type of activated G protein. When the Gs protein is activated, it will cause the intracellular cAMP concentration up-regulated by adenylate cyclase activation, further activate protein kinase A, and finally promote the phosphorylation of some target proteins, which may be involved in, for example, dopamine signaling. Important physiological and biochemical processes such as conduction, glucose regeneration, vasodilation, cell division, and egg maturation [2-6]. The CatchPoint® cAMP Fluorescence Detection Kit uses the principle of immunocompetence to measure cAMP concentration (Figure 2). It only needs to be eluted in one step, and the reaction substrate can be placed for 10 minutes after being added, and can be extended to 24 hours before detection. The signal is stable. Sensitive.

Amino Acids

Amino acids are carboxylic acids containing amino groups. Amino acids are the building blocks of protein for animal nutrition. Proteins in living things are made up of 20 basic amino acids.

Amino acids in human body through metabolism can play the following roles: (1) synthesis of tissue protein; (2) into acids, hormones, antibodies, creatine and other ammonia containing substances; (3) to carbohydrates and fat; (4) oxidation into carbon dioxide and water and urea, produce energy.

Alanine for the synthesis of Alitame, intermediate amino acid Serine, nonessential amino acid Arginine

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