(a) principle

When the radioactive element-labeled antibody (or antigen) is precipitated with the corresponding antigen (or antibody), the precipitation line is confirmed by autoradiography. The so-called auto-development is the radiation emitted by the labeled isotope in the composite during the enthalpy change, which acts on the silver halide crystal of the photosensitive material to produce a latent image, and then develops the "image". In this way, the precipitation line which is invisible to the naked eye can be detected, thereby increasing the sensitivity of the reaction, and this method can be applied to all reactions in the gel.

(2) Materials

1. Radioisotope labeled antigen or antibody

2, X-ray film or full color film

3. Developer and fixer

4. Other convective immunoelectrophoresis

(3) Operation method

1. Gel plates were prepared by convective immunoelectrophoresis and perforated.

2. Add antigen to the cathode end of the sample and add antibody to the end of the anode. When adding the sample of the test antibody, the labeled antigen (2 000~3 000 cpm) is quickly added and mixed, and the antigen is not absorbed after the antigen is absorbed, so as to avoid false positive due to the lag surface of the original label.

3. 4 mA for electrophoresis current 2.6 cm × 7.5 cm.

4. After electrophoresis, the plate was immersed in 3% HCl for 8 hours, and the solution was changed twice.

5. Rinse overnight with running water.

6. Dry at room temperature or blow dry.

7. Exposure cut the film into the same size as the plate in the dark room, then close the surface of the gel to the gel surface, press a glass plate on the film, wrap it tightly with black cloth, tighten the rubber band, expose the time and use the isotope and save Related to the period. 181I, 5h~10h within one week; 24h within 1~2 weeks; 48h within 2~3 weeks. 125I, 10h in 2 months.

8. Flush the full-color film punching method, wash with water, develop at 20 ° C for 10 min, wash, fix, wash, and dry, observe the results.

(4) Result determination

A clear black image was formed between the antigen-antibody wells and was positive.

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