HIV will not be able to "latent"
Release date: 2015-08-07 The HIV in situ analysis technology has once again made a breakthrough. At the International AIDS Conference held last week, American scientists demonstrated their new detection technology and test results. This probe called "Molecular Microscope" can accurately detect the hidden places of HIV inside and outside the cell. Source: Technology Daily Vital Signs Monitor,Pulse Oximeter,Oxygen Concentrator,Patient Monitor,valve inflatable extension tube Anesthesia Medical Co., Ltd. , https://www.medicaldiverse.com
Ricardo Pul, deputy director of the Vaccine Research Center of the American Institute of Allergy and Infectious Diseases, said that the new technology of this molecular microscope is magical, and its super powers can fully understand the traces of HIV in any cell. Help clear the mystery of the long-term survival of HIV, so that it can be completely removed from the body.
New technology is almost undisturbed
The in situ analysis techniques used to detect HIV in tissues currently face common problems. These detection techniques, whether using fluorescent substances as markers or radioactive materials for labeling, are often difficult to accurately locate the location of HIV in tissue samples, and it is often difficult to separate surrounding cellular material with target analytes, such as HIV RNA and DNA. The difference is open. These markers misidentify the cell tissue as a virus and cause background interference in the analysis of the results.
According to the "Science" magazine website, detailed pictures of HIV obtained in different organizations of monkeys at the conference show that the new technology has received almost no interference. Jack East, an immunologist at the National Cancer Institute's Frederick National Laboratory, has teamed up with the American Advanced Cell Diagnostics Inc. (ACD), which has an RNA microscope, to develop this new technology that can detect tissue separately or simultaneously. DNA and RNA in HIV. Thanks to ACD's unique probe design patent, RNA Microscopy is the most advanced RNA detection technology tool, enabling visualization and quantification of individual RNAs in situ, enabling simultaneous signal amplification and reduced background interference for any tissue Any gene. The molecular microscope for detecting HIV was developed on the basis of RNA microscopy.
Both DNA and RNA consist of complementary pairs of nucleotides. Traditional methods of capturing genetic material use long strands of nucleotides called oligomers to find and pair with DNA or RNA strands in tissue samples. These oligomers carry markers, and when it detects the target, the marker signals and takes pictures, and the researchers can find the location of the viral genetic material in the tissue sample from the image. But these oligomer molecules are too long, they occasionally make mistakes, and when combined with other cellular substances, they ignore the target sequences to be detected.
Principle of molecular microscopy
East's new technology includes a more sophisticated probe system that completely eliminates the misunderstandings caused by oligomers. The basic principle of the technique is that the oligomers are first cut into two equal parts, and then the two equal parts are sent to the sample to find the target sequence. Only when the separated two segments stay near the target detection sequence can they After successfully pairing with the target sequence, reconnect them. This means that the two segments of the oligomer can only be paired and re-engaged only when they encounter HIV, and other cellular substances can no longer cause interference.
The HIV virus itself is an RNA virus, but it is converted into a DNA form so that it can "sneak into" the human chromosome at any time. In collaboration with virologist Jeffrey Lvivson, Eastbour has successfully developed a DNA microscope that visualizes HIV DNA. These latent viral precursors are incorporated into human cells and remain hidden for decades before being attacked by the immune system or antiretroviral drugs. An important reason why antiretrovirals are unable to eliminate AIDS infection and cure AIDS patients is These large numbers of cells that "hidden" the viral precursors.
Never let go of any virus
East, Livson and colleagues injected scorpion AIDS into some monkeys and then analyzed in situ the many tissues in these monkeys. The results show that RNA microscopy and DNA microscopy clearly distinguish between latent HIV precursors (viral DNA), viral RNA, and extracellular viruses. East said: "I firmly believe that our new technology will not let go of any virus, it perfectly combines sensitivity and specificity."
These new molecular microscopes can overcome several major obstacles to the cure of AIDS. The first major obstacle is the inability to detect HIV in the plasma of AIDS patients receiving antiretroviral therapy, so it is difficult for researchers to assess the specific effects of some new AIDS therapies, and new microscopy techniques will be a powerful complement to overcoming existing technological barriers. Another major obstacle is the inability to know exactly where the virus precursors are hidden in the body. New technologies can uncover this long-standing mystery, help to significantly reduce the number of cells infected with HIV, and treat patients more specifically.