Biosafety cabinet for laboratory biosafety main equipment
one, Â Biosafety cabinet overview
(1) Concept: The biological safety cabinet ( BSC ) is used to protect the operator, the laboratory environment and experimental materials when operating infectious materials to avoid exposure to infection during operation. Negative pressure exhaust cabinet designed for aerosols and spills.
(2) Choice of biological safety cabinet
Biological safety cabinet | Type of protection | Protected object |
Level I | Second, third and fourth pathogenic microorganisms; volatile radionuclides/chemicals | Operator, environment |
Class II A1 | Second, third and fourth pathogenic microorganisms | Operator environment and samples |
Type level â…¡ A2 | Two or three types of pathogenic microorganisms; trace volatile radionuclides / chemicals | Operator environment and samples |
Class II B1 | Two or three types of pathogenic microorganisms; small amounts of volatile radionuclides/chemicals | Operator, environment and sample |
Type level â…¡ B2 | Second, third and fourth pathogenic microorganisms; volatile radionuclides / chemicals | Operator, environment and sample |
Level III | One, two and four types of pathogenic microorganisms; volatile radionuclides/chemicals | Operator, environment and sample |
1. Class I Biosafety Cabinet - Class I Biosafety Cabinet provides protection for people and the environment, but does not provide product protection.
Uses: Class I biosafety cabinets are used as special sealing equipment (such as centrifugation, harvesting equipment or small fermentation equipment) or processes that may generate aerosols (such as garbage dumping, aeration culture or tissue agitation). It can also be used to operate radionuclides and volatile toxic chemicals. It has been replaced by a medical air isolation device and has higher safety protection performance.
2. Class II biosafety cabinets - Class II biosafety cabinets provide protection for people, the environment and products. Uses: Perform operations related to levels 1, 2, and 3 of biosafety.
3. Class III Biosafety Cabinet - Class III Biosafety Cabinet is a hermetic structure without an open viewing window; providing maximum protection to the environment, products and operators. Use: To operate a microbiological pathogen of level 4 biosafety.
Second, the use of biological safety cabinet
(1) Personal protection requirements: 1When using the biosafety cabinet, you must wear personal protective clothing and gloves (the gloves should be placed on the outside of the isolation garment). 2 It is best to wear a mask and a hat, and wear a protective eye mask or mask as needed. 3 Before working, the operator should adjust the height of the stool to ensure that his face is above the opening.
(2) Principles for placing items in the biosafety cabinet: 1 Place in the direction from the clean area to the contaminated area. 2 Place all items as far as possible from the front grille, near the rear edge of the console. 3 Place large-sized items such as waste bags, trays for discarded straws, and suction filter bottles on one side of the cabinet. 4 front grille can not be blocked by experimental records, equipment or other items
(3) Operation process of biological safety cabinet : open the power switch of the exhaust fan outside the biological safety cabinet - slowly open the operation window, the window opening distance is 200mm or the set value, the general biological safety cabinet has a window limit alarm - 10 minutes later Start the experiment operation - pay attention to the disinfection and cleaning work of the biological safety cabinet after the operation is completed.
(4) Precautions for the use of biological safety cabinets - 1 Safety cabinets can only be used when they are working properly. 2 Slowly enter the front opening with both arms and wait approximately one minute in the biosafety cabinet to allow the cabinet to be processed before the cabinet is adjusted. 3 Waste should be disposed of in the processing container inside the biosafety cabinet to prevent the arm from moving quickly and frequently in and out at the front opening to avoid interference with the airflow. 4 Try to avoid contaminated items entering the clean area. 5 When working in the biosafety cabinet, text work is not possible. All work in the 6 cabinets should be carried out in the middle or rear of the work surface and can be seen from the viewing panel. 7 The forward style grille must not be blocked by notes, droppers, or other materials, otherwise it will interfere with the airflow and may contaminate the material and expose the personnel. 8 Cleaning and Disinfection: The surface of the safety cabinet should be cleaned with a suitable disinfectant after each work and at the end of the day. The fan of the 9 cabinets must be operated for 5 minutes before the start of work and after the work is completed.
Third, the ultra-clean workbench
The ultra-clean workbench is designed to protect test samples and protects the test sample from dust or bacteria by laminating air blown through the work area. Infective materials cannot be handled in the ultra-clean workbench because their gases are directly discharged under positive pressure. Once the infectious sample is placed in the work area, laminar air will create a hazard by blowing air with microbial media to the staff at the front desk.
The difference between biological safety cabinet and ultra-clean workbench
name | structure | gas pressure | Gas emission | Protected object |
Biological safety cabinet | complex | Negative pressure | Emission after purification | operator  sample  surroundings |
Ultra-clean workbench | simple | Positive pressure | Direct discharge | sample |
In addition to understanding the performance indicators of the safety cabinet, the best and quickest way to identify the safety performance of the safety cabinet is to know whether the biosafety cabinet you purchased has obtained the following major international safety cabinet certifications: NSF49 (US Biosafety Cabinet Standard) - Class II biosafety cabinet), EN12469 (European Biosafety Cabinet Uniform Standard), etc.
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brand introduction:
Jinan Xinbeixi Biotechnology Co., Ltd. Â
Anti-Infective IV Solutions
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Shandong Qidu Pharmaceutical Co., Ltd. , https://www.qdyypharma.com
