Among the chemical pesticides used in the production of fruits and vegetables, the most varieties and the largest amount, the most likely to cause people to be poisoned are organophosphate insecticides. At present, the CSY-N16 pesticide residue detector produced by Shenfen Instruments can quickly detect pesticide residues in vegetable products, as follows:

First, CSY-N16 pesticide residue detector method

1. Normal working conditions of the instrument.

(1) Room temperature 25-35 ° C (water bath at room temperature below 25 ° C).

(2) The indoor relative humidity is not more than 85%.

2. Reagent preparation.

1 Buffer: Pour the reagent in the buffer reagent bag, dissolve in 500ml distilled water, dissolve and mix.

2 Substrate: Add 13 ml of distilled water to the bottle labeled as substrate. Store in cold storage at 2~4oC.

3 Color developer: do not need to be placed in the refrigerator (2~4oC), do not freeze and freeze.

4 Enzyme reagent: The enzyme reagent has been formulated into a solution and can be used directly. It is usually stored under refrigeration in 2~4oC environment. Do not freeze until icing! ! ! .

3. Preparation of test solution.

(1) Picking 2-2.5Kg edible dishes from the field, divided into 3 parts, 1 record, 1 part for re-examination, and 1 part for testing. Take the vegetables with clean surface, accurately weigh 2g with the balance, take the leaves of the leaves, and cut the slices of fruits and vegetables such as tomatoes and cucumbers.

(2) Cut the leaves, melon meat, etc. into 1cm square, place in a small beaker, add 10ml extract to immerse, leave it at room temperature for 10min, shake the beaker every 3min.

(3) Pour the extract from the beaker into the test tube. After a little sedimentation, absorb 2.5 ml of the supernatant with a pipette and transfer it to another test tube.

4. Instrument operation and testing.

3. Test sample test:

(1) Blank control measurements:

1 Take 2.5ml of the control sample in the cuvette;

2 Place the cuvette in the designated channel;

3 Press the “Control” button and the measurement time is displayed at the bottom of the screen;

4 After the test is finished, the instrument automatically displays the test result â–³Ao of the control sample.

[Remarks]: a When the color development time is 1 min, when the blank control value is between 0.15 and 0.3, the experiment can be continued;

b When the blank control value is <0.15, the blank control should be redone. If the blank control value <0.15 is repeated multiple times, the enzyme reagent must be replaced.

c When the blank control value is >0.3, the enzyme solution should be diluted appropriately and the blank control should be repeated.

d When the color development time is 3 min, the control value should be ≥ 0.3. When <0.3, the enzyme reagent was changed.

(2) Sample measurement:

1 Take 2.5ml of the sample to be tested in the cuvette;

2 Place the cuvette in the designated channel;

3 Press the “sample” button and the measurement time is displayed at the bottom of the screen;

4 After the test is finished, the instrument automatically displays the test result â–³Ai of the specified channel test sample.

[Judgement criteria]

(1) The inhibition rate of the sample is between 40% and 50%, which is a suspected pesticide residue exceeding the standard;

(2) The inhibition rate >50% is the pesticide residue exceeding the standard, indicating that the pesticide residue toxicity of the tested sample may exceed the safety defined standard. It is recommended to use the instrumental analysis such as gas chromatography for further confirmation.

【Precautions】

1 After adding the substrate, it should be mixed quickly and tested immediately.

2 The test solution used has different degrees of damage to the skin, so please take good protection when using it. In case of accidental skin contact, dry it immediately and rinse with plenty of water.

3 Please check the reagents in the 2~4oC refrigerator.

Second, matters needing attention

1. During the preparation of the test liquid, it should be shaken every 3 minutes during the leaching, and it will be affected by shaking and not shaking. The leaching time should be guaranteed for 10 minutes, and the shortening time has a great influence on the results.

2. It is recommended to use the same cuvette (wash 2 times with distilled water before each use, then dry and wipe the glossy surface with a mirror paper). Using different cuvettes has a great effect on the results. A batch of samples can be detected at the same time, sharing a control, which is also conducive to shortening the working time.

3. The reaction time of the enzyme solution and the color developer and the extract is 15 min, the reaction time is different, and the results are different. The longer the time, the higher the sensitivity.

4, enzymes, color reagents can not be added and added, otherwise the impact on the results. In the process of testing the samples of vegetables, it should be taken seriously and according to the operating procedures. It is recommended that the samples with inhibition rate ≥50% or more should be further qualitatively and quantitatively analyzed by chromatography or mass spectrometry to determine whether the pesticide residues are true. Excessive.

5, onions, garlic, leeks, taro, medlar and other vegetables, using enzyme inhibition method for rapid measurement, the possibility of false positives is extremely large, and the more spicy and spicy garlic varieties, the higher the false positive frequency, but not A false negative problem was found during the test.

6, purple red eggplant, tomato, red pepper, red amaranth, loofah contains natural pigments, and juicy, using enzyme inhibition method for rapid measurement, the results are not affected by pigment and juice. For the winter vegetables, spinach, celery, etc. in the green leafy vegetables, the results of the enzyme inhibition test are normal, and the possibility of false positives and false negatives is not significant.

7. After adding the substrate, it should be mixed quickly and tested immediately.

8, water spinach, cabbage, beans, etc. are mandatory inspection items, because these kinds of vegetables or easy to residue pesticides, or the use of pesticides, the number of times, the density. However, the detection of other vegetable types cannot be ignored.

9. The original records of sampling, test results, etc. should be prepared, and it is best to use computer to print, which is convenient for the management of archiving and inspection.

10. The test solution used has different degrees of damage to the skin. Please use it when using it. In case of accidental skin contact, dry it immediately and rinse with plenty of water.

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