(1) Reagents and materials The reagents used below are analytically pure secondary waters unless otherwise specified. Water is in accordance with GB/T 6682
1 neomycin standard product 2 dipotassium hydrogen phosphate 3 potassium dihydrogen phosphate 4 sodium hydroxide 5 protein biochemical reagent 6 agar biochemical reagent 7 yeast powder biochemical reagent 8 beef extract biochemical reagent 9 glucose 10 sodium chloride (11) sterile phosphate buffer Solution (0.1 mol/L, pH 8.o) 16.73 g of anhydrous dibasic potassium phosphate and 0.523 g of anhydrous potassium dihydrogen phosphate were accurately weighed, dissolved in water and diluted to 1000 ml. Sterilize at 121~C for 20 minutes, and set aside.
(12) The neomycin standard solution accurately weighs the appropriate amount (not less than 20mg) of neomycin standard, dissolve it with sterile phosphate buffer solution (o.1mol/l pH 8.o), and dilute to a concentration of 100bg/m1. The standard stock solution is vacuum filtered and stored in a 4~C refrigerator for 4 weeks.
(2) Instruments and equipment 1 flat bottom double disc diameter of about 90mm, height 16 ~ 17mm
2 Oxford Cup stainless steel, height 10.0mm, outer diameter 8.0mm inner diameter 6.0mm
3 vernier caliper accuracy o. 02mm
4 vortex mixer 5 vacuum pump 6 centrifuge 7 spectrophotometer 8 thermostatic electric hot water bath 9 analysis balance sensitivity o. 0001g
10 electrothermal constant temperature incubator (3) determination step 1 preparation of bacterial suspension S. epidermidis 26069 type was continuously passaged on fresh medium I for 3 times, then rinse the slant culture with an appropriate amount of 0.85% NaCl sterilization solution and dilute Inoculum suspension. At a wavelength of 560 nm, the light transmittance of the bacterial suspension is controlled at about 80%. The bacterial suspension should be prepared fresh.
2 Preparation of double dish The bottom layer was added with 10 ml of melted medium I in a double dish, uniformly flattened, and allowed to solidify on a flat surface.
The bacterial layer is pre-melted and maintained in the medium II of 48 ° C to add an appropriate amount (usually o. 2 ~ o. 5m1) of the newly prepared bacterial suspension. After thoroughly mixing, 4.0 mi was added to each of the cultured double dishes containing the bottom layer. The two sides of the cultured two-disc are tilted and rotated in a circular motion to evenly spread the culture medium, and to be solidified. Prepare a double dish on the day of use. The diameter of the zone of inhibition obtained from the standard working fluid with reference to the concentration should be (18 soil 2.4) mm.
3 standard curve preparation using o. 1mol/L phosphate buffer solution (pH 8.o) diluted neomycin standard stock solution into a concentration of 2.4bg / ml, 1.2 / Lg / m1, 0, 6gg / m1, 0. 3 / lg / ml , 0.15bg/m1 series of standard working fluid, to o. The standard solution of 6ug/ml is the standard reference concentration working solution. The diameter of the inhibition zone is determined according to the microbial agar diffusion method. The diameter of the inhibition zone is plotted on the coordinate paper. The logarithm of each concentration is plotted on the ordinate and the average diameter of the inhibition zone is The correction value is a standard curve drawn on the abscissa.
4 Extract and weigh (5±.05) g sample, put 50ml plastic centrifuge tube, add 10mlo. 1mol of phosphate buffer solution (pH 8.O), mixed on a vortex mixer for lmin, O ~ 4 ~ C for 30min, put into a 100 ~ C water bath for 5rain, take out cooling, 5000r / mill centrifugation l5min, take The supernatant was adjusted to pH 8.0 with a 1 mol/L NaOH solution and used as a sample solution for microbiological determination.
5 Determine the separation of 3 oxford cups and standard reference concentration working solution for each double-disc interval, and add the sample solution to the other 3 oxford cups. Repeat 3 double-discs for each sample and culture for 18-20 hours at 32-35~C. The diameter of the inhibition zone was measured, and the drug content was determined from the standard curve.
6 Blank test Parallel operation using the same measurement procedure except for the absence of sample (4) Sensitivity and recovery The detection limit of this method in chicken tissues is 500 ug/kg.
The recovery range at the concentration level of 500ug/kg is 80% to 110%

Huaiyang Wanyuan Garlic Foods start business in 2005.Which is a manufacturer and exporter of Vacuum fried,Fried and Dehydrated vegetables.We also got a patent for VF products.

The Color of Vaccum Fried Carrot Granules is same as fresh Carrot.

Crispy VF carrot Granules can be eat direcely as snacks ,and mixed with other VF vegetables.

Vacuum Fried Carrot Granules

Vacuum Fried Carrot Granules

Huaiyang County Wanyuan Garlic Foods Processing Industries Co.,Ltd , https://www.wanyuangarlicfood.com