(1) Production of mother plants

The tissue separation method is simple and easy to carry out, the variation of the offspring is small, and the excellent characteristics of the strain can be preserved. It is a commonly used strain separation method in production. Mushrooms should be selected for typical appearance, moderate size, less scales on the surface of the mushroom cover, and pure white heads with no disease and pests. The picking of the mushroom should be in the late stick or fusiform stage where Coprinus comatus grows.

The mother culture medium can choose PDA medium. The following formula can also be used: 600g of wheat, 100g of fresh fruit body, 20g of glucose, 3g of peptone, 0.5g of potassium dihydrogen phosphate, 1g of dipotassium hydrogen phosphate, magnesium sulfate 1 Grams, 1 g of calcium sulfate, 25 g of agar, and 1000 ml of water. The production process of the parent culture medium and the aseptic separation technology are the same as those of other mushroom production techniques and are not described.

(2) Original production

Coprinus comatus used in the production of the original varieties of wheat seeds, the preparation method is as follows: soaking wheat for 24 hours or so, to white grain, rinse with water and boil for 20-25 minutes, remove and drain water and add 1% gypsum powder, 20% of straw mash fermentation material, then bottling, sterilization, sterile inoculation and cultivation. The mycelium of this formula grows vigorously and grows fast. In a 25°C environment, the 750 ml bacteria culture bottle can cover the bottle for 12-15 days.

(3) Production of cultivars

Coprinus comatus cultivars are mostly prepared from cotton hulls and wheat straw fermentation materials. The formula is as follows: straw, cow dung, fermented material 33%, bran 10%, cottonseed skin 50%, corn flour 5%, lime 2%. The wheat stalks were first crushed by adding 20%-30% of cattle manure, adding 2% of gypsum, and accumulating and fermenting for 5-6 days. Then, other raw materials were added, and the bag was sterilized after being fermented for 48 hours, and was inoculated and cultured aseptically. This formula has the advantages of faster fermentation and stronger mycelium than simply using fermentation material and pure cottonseed skin.

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