Name:Histone H3K9me3 (H3K9 Trimethyl) Polyclonal Antibody

Goods number: A-4036

Application: ChIP, ChIP-Seq, ELISA, IF, IHC, IP, WB

Clone: ​​Polyclonal

Whether with a mark: no mark

Host: Rabbit

Isotype control: IgG

Purification: affinity purification

Reactive species: widely available species, human, mouse, rat

Concentration: 1 mg/ml

Description: Rabbit polyclonal antibody is a synthetic peptide directed against K9 trimethylated histone H3, chip scale

Immunogen: A synthetic methylated peptide corresponding to human histone H3 K9 residue buffer solution: PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Storage: short-term -20 ° C, long-term -80 ° C, to avoid repeated freezing and thawing.
H3K9me3 antibody; H3K9m3 antibody; HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3

Dilution ratio when used:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
ChIP: 1:50 - 1:200
ChIP-Seq: 1:50 - 1:200

background:

In eukaryotes, the regulation of chromatin structure plays an important role in transcriptional regulation. Nucleosomes, composed of DNA entangled on eight core histones (H2A, H2B, H3 and H4 each) are the basic structural units of chromatin. The amino terminus of the core histone undergoes various post-translational modifications including acetylation, methylation, phosphorylation, and ubiquitination. These modifications occur under a variety of stimuli and directly affect the intimacy of chromatin and transcription factors, thus affecting gene expression. In most species, the site of histone H2B major acetylation is Lys5,12,15,20. The major acetylation sites of histone H3 are LYS9, 14, 18, 23, 27, and 56. Lys9 acetylation of histone H3 appears to have dominated protein deposition and chromatin assembly in some organisms. Phosphorylation of Ser10, Ser28 and Thr11 of histone H3 is closely related to chromosome agglutination during mitosis and meiosis. THR3 phosphorylation of histone H3 is highly conserved in many species and is catalyzed by the kinase haspin. Immunohistochemically stained phosphorylation-specific antibodies in mammalian cells revealed phosphorylation and late dephosphorylation of Thr3 in histidine H3 in pre-mitotic phase.

references:

Tyler CR et. al. (July 2015). Developmental exposure to 50 parts-per-billion arsenic influences histone modifications and associated epigenetic machinery in a region- and sex-specific manner in the adult mouse brain. Toxicol Appl Pharmacol.

Wiegreffe C et. al. (July 2015). Bcl11a (Ctip1) Controls Migration of Cortical Projection Neurons through Regulation of Sema3c. Neuron. 87(2):311-25.

Kumar P et. al. (March 2014). Histone deacetylase inhibitors modulate the transcriptional regulation of guanylyl cyclase/natriuretic peptide receptor-a gene: interactive roles of modified histones, histone acetyltransferase, p300, AND Sp1. J Biol Chem. 289(10 ):6991-7002.

Sui WG et. al. (January 2014). ChIP-seq analysis of histone H3K9 trimethylation in peripheral blood mononuclear cells of membranous nephropathy patients. Braz J Med Biol Res. 47(1): 42-9.

Trapphoff T et. al. (December 2013). Chronic exposure to a low concentration of bisphenol A during follicle culture affects the epigenetic status of germinal vesicles and metaphase II oocytes. Fertil Steril. 100(6):1758-67.e1.