ACE CN-ES LC column

• New stationary phase technology for UHPLC and HPLC separations • Alternative polar selectivity for method development • High column efficiency 2μm, 3μm, 5μm and 10μm particles • Ultra inert for optimum performance and reproducibility

ACE CN-ES LC column

Target analyte

• Polar and non-polar analytes
• Analyte with double and triple bonds
• Hydrophobic analytes
• Poor polar molecules remain on traditional CN bonded phases

Recommended separation

• a mixture of highly polar, polar and non-polar analytes
• Suitable for NP and RP separation
• Traditional CN bonded phase shows stability/poor separation
• Typical C18 columns do not provide adequate separation applications
• Orthogonal phase in method development

ACE CN-ES LC column provides alternative selectivity

Samples: 1) Metronidazole 2) Benzyl alcohol 3) Hydrochlorothiazide 4) Vanillin 5) Methylparaben 6) 1,2-Dinitrobenzene mobile phase: A = 0.1% formic acid (dissolved in water) B = 0.1% formic acid (dissolved in 90:10 methanol / water)
Gradient: 3 - 100% B in 5 minutes
Column size: 50 x 2.1mm

Flow rate: 0.60ml/min
Temperature: 40 ° C Wavelength: 254 nm

Why can an ACE CN-ES LC column provide an alternative selectivity?
â–  Combining polar CN groups with extended spacers provides a similar retention to C18 columns â–  Compared to traditional CN bonded phases with short-chain alkyl spacers (usually C3/propyl) Extended spacer technology for additional column life

ACE CN-ES offers similar retention to C18 columns

Separation of neutral compounds under RP conditions

Samples: 1) uracil 2) dimethyl phthalate 3) toluene 4) biphenyl 5) phenanthrene

Mobile phase: 60:40 acetonitrile / water column size: 150 x 4.6mm

Flow rate: 1.00ml/min

Temperature: 22 ° C

Wavelength: 254nm

Alternative selectivity using methanol or acetonitrile

Advantages of multi-mode interaction on separation

Samples: 1) 1,3-dihydroxybenzene 2) catechol 3) hydrochlorothiazide 4) oxyenolol 5) salicylic acid 6) myricetin 7) piroxicam 8) 1,2-dinitrobenzene 9) Tommetine 10) 1-naphthol 11) piperine 12) diflunisal
13) propylbenzene 14) 1,2,3-trichlorobenzene

Mobile phase: A = 0.1% formic acid (dissolved in water) B = 0.1% formic acid (dissolved in acetonitrile)

Gradient: 3 - 100% B in 10 minutes
Column size: 100 x 2.1mm

Flow rate: 0.60ml/min

Temperature: 40 ° C

Wavelength: 210nm

Fast gradient of NSAIDs

Samples: 1) 2-acetaminophen 2) Acetanilide 3) Salicylamide 4) Aspirin 5) Phenacetin 6) Salicylic acid mobile phase: A = 0.1% formic acid (dissolved in water) B = 0.1% formic acid (dissolved in methanol)
Gradient: 5 - 38% B in 3.75 minutes, keep 38% B until the 5th minute Column size: 50 x 2.1mm

Flow rate: 0.60ml/min

Temperature: 40 ° C

Wavelength: 240nm

Enhanced selectivity on UHPLC

Separation of steroids by UHPLC

Samples: 1) Cortisone 2) Corticosterone 3) 11α-hydroxyprogesterone 4) Cortisone-12-acetate 5) 11-ketoprogesterone 6) β-estradiol 7) 17α-estradiol 8) 17α-ethynyl estradiol 9) estrone mobile phase: A = 0.1% formic acid (dissolved in water) B = 0.1% formic acid (dissolved in acetonitrile)

Gradient: 25 – 52.5% in 5 minutes B
Column size: 50 x 2.1mm

Flow rate: 0.40ml/min

Temperature: 40 ° C Wavelength: 260 nm

ACE CN-ES is compatible with normal phase conditions

Comparison of retention with substituted aromatics under NP conditions

Samples: 1) butylbenzene 2) methyl benzoate 3) nitrobenzene

Mobile phase: 90:10 heptane / ethyl acetate column size: 150 x 4.6mm

Flow rate: 1.00ml/min

Temperature: 22 ° C

Wavelength: 254nm

Product availability and specifications

YT-H008

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