Several common vegetable seed dyeing methods and criteria
Cefuroxime Sodium,Vitamin Injection,Powder Injection Pharmaceutical Products for Human Co., Ltd. , http://www.beautyinjectable.com
November 08 21:01:11, 2018
There are many types of vegetable seeds, but from the perspective of the internal structure of the seeds and the morphology of the embryos, the seeds of the same “genus†are basically the same, but there are differences in the size of the seeds and the external morphology. Before the dyeing of vegetable seeds, the following work needs to be done: First, seed softening treatment is performed, that is, the seed is placed between two layers of warm sand cloth according to two repetitions of 100 materials. Second, the softening treatment time is due to the size of the seed and the thickness of the seed coat may be, usually between 8-12 hours (at room temperature conditions), if the urgent request for this batch of seeds can be heated 30-40 °C About 3-5 hours, or without soft treatment, the embryo was cut directly with a knife, but the effect was not good. Third, the dyeing method (a) Lily Branch: onion, leek 1. Treatment: The softening of the seed in the mouth under the bud cut, tap the seeds gently with tweezers, so that the embryo exposed. 2. Staining: staining with 1% tetrazolium solution at 30°C for 60-80 minutes. 3. Judgment criteria: Germs are all germinated. Knife cut the wound is white, all the red embryo germination. The embryos are not stained or the embryos are light and pale pink and do not germinate. (B) Solanaceae: pepper, eggplant, tomato 1. Treatment method: Cross a knife at the germination mouth of the softened seed, press gently with a forceps to expose the embryo, and acupuncture with a needle on the seed puncture. 2. Staining: 0.2-1% tetrazolium solution was stained at 40°C for 40-60 minutes. 3. Seed internal observation: The acne method should observe whether the inside of the seed coat is red through the seed coat, and observe the light outside the seed directly after dyeing. Observe the dark seed coat after decoloring with a transparent agent (lactic acid phenol). After dyeing, the seeds were rinsed with water, and then the transparent agent was dripped for 3 hours. The seed coat became lighter and the inside of the seeds could be observed. 4. Judgment criteria: 1 Pepper: The embryos are all stained red, and the cut-off part of the knife-cut wound is white, but the embryo is completely stained with red for germination. The embryos are pale and pink, and the embryos are not stained or lightly shaded. 2 Eggplant: When observing the eggplant dyeing, the embryos need to be extruded out of the material and the embryos are fully stained in red; the knife-cut wounds are broken in white, the embryos are colored red and germinate, and the pigments are pale, pink, embryos. All non-stained parts do not germinate. 3 Tomatoes: Embryos are completely reddish to germinate, embryos are partially stained, embryos are light pink, and embryos are not stained for germination. (3) Cucurbitaceae: Cucumber, Pumpkin 1. Treatment method: After softening the seeds, peel the outer seed coat and the transparent seed coat, and cut a small part at the other end away from the germination hole to expose the two cotyledons. The gap is split with a needle or tweezers. The embryo is left intact on a leaf and placed in a plate for staining. 2. Dyeing: 1% tetrazolium solution, staining at 80-40°C for 60-100 minutes. 3. Discriminating criteria: All cotyledons and embryos were dyed red; cotyledons had small white spots, and the rest of them were dyed red for germination. The connective parts of cotyledons and embryos were white; embryos were not pigmented, and 1/3 of the cotyledons were not stained; seeds were pale pink; seeds were not stained and did not germinate. (d) Cruciferae: Chinese cabbage, radish, rape 1. Treatment method: Seeds are softened and peeled with tweezers. 2. Staining: 1% tetrazolium solution, stained at 30-40°C for 60-100 minutes. 3. Discrimination criteria: Cotyledons and embryos fully stained: only germinated at the end of the radicle. The cotyledons had small white spots, and the radicles and cotyledons of the embryos were not stained under the connection; more than 1/3 of the cotyledons were not stained or dyed light pink, and all were not germinating. The above are some common methods for tetrazolium staining. It is accurate, reliable and fast. It is a good judgement for timely seeding and seeding of seeds, and it is still concluded that the actual germination rate is high and the error is within 5%. Strict operating methods and judgment standards still yield values ​​that are very close to the actual germination rate.