Two new methods of hybrid propagation of gastrodia hybrids
Tips Racks,Pipette Tips Box,Racks For Pipette Tips Rong Tai Biochemical Engineering Co., Ltd. , http://www.shpipette.com
December 10 21:17:19, 2018
Gastrodia and arrow bud maternal pot breeding method (to Yidu red gastrodia as an example) 1, potted nursery: At room temperature stability above 15 °C before and after Ching Ming Festival, pick fresh and robust, bud top full, no injury, weight 100-150 The wild red red gastrodia, the arrowhead bud body (marked wild red) and the plain red house gastroid shoot bud mother (marked red) are planted in the shabby plastic washbasin or enamel washbasin, respectively, in each of the pots. Place the arrowhead bud body 4 (strain), Arrowhead (parrot brother mouth) upward from the basin edge 3-5 cm, cover 5-10 cm thick moist river sand. About 15-20 days of culture, when the temperature reaches 19 °C or more, Tianma twitches and flowers. 2. Pollination and Breeding: After artificial flowering and flowering of Tianma, artificial cross-pollination and cross-pollination based on inflorescences are conducted one by one. Specific operation: Holding a small Plastic Plate in the left hand, and fixing the receptacle marked with wild red gastrodia, the right hand holding the large Needle peels the outer flower tube of the flower, exposing the column, picking the cap with the needle tip, and sticking the light yellow pollen stick on the sticky disk. Tip, lightly placed in a plastic tray. Follow the method of sticking the pollen stick of the red flag of the family Gastrodia elata directly to the sticky disk of the wild red gastrodia fruit and gently press it; move the needle tip to pick the wild red gastrodia elata pollen group on the plastic disc to the sticky disc of the red gastrodia elata flower. Light pressure Serve. After about 15 to 25 days of natural growth, the pollinated flowers gradually enlarge into pod fruits (pollen seeds). When the capsule is hard and soft, it will be timely harvested when the crack is about to occur. One and a few adjacent fruits will be harvested and stored in a refrigerator at 5-10°C or with the income. Gastrodia hybrid sand fruit bed rapid propagation method (take 1 square meter as an example) 1, material preparation: 1 fruit powder species: 3 kinds of leaves germinating bacteria, into a jujube grain size, take 20 hybrids of Gastrodia, shake out the inside of the fruit Pollen seeds, mixed with a little dry sawdust, and then mixed with the leaves germination bacteria, let stand 1 night. Two branches of honeydew 3 strains of honeysuckle, respectively, into a single branch of date size. 3 about 100 kg of clean sand in the river. 4 25-30 kilograms of fresh and mixed trees. Among them, there are 14 thick wood segments with a diameter of 4-8 cm and a length of 30 cm, 24 fine wood segments with a diameter of about 2-3 cm and a length of 20 cm. 0.2% carbendazim solution was soaked in water for 24 hours respectively. After draining slightly, two phosphorus fish mouths were cut on each side of each wood section, and one branch of honeybee ring fungus was planted in the mouth of scales (amount of use was about 1 bottle). ); about 1 cm in diameter, length of 4-5 cm branches about 3 kg. 5 dry leaves 4 kg, 0.2% carbendazim solution soaked wet. 6 half insecticide spirit bottle, double-effect powder half a pack. 2. Sand bed cultivation: The red brick or cement hollow brick is placed in a rectangular frame with a width of 80 cm, a length of 125 cm and a height of 15-20 cm in the indoor ground plane. A 5 cm thick moist river sand is placed on the bed as the first floor. Tile 1 layer of wet leaves for the second floor. The third layer spreads evenly half a bottle of honey ring mushroom species. The fourth layer evenly spreads fruit powder. The 5th layer evenly spread half a bottle of honey fungus. The 6th floor is tiled with 1 wet leaf. The 7th floor evenly placed coarse wood and fine wood sections. The 8th layer of coarse wood and fine wood were uniformly planted with 1 bottle of honeydew species. The 9th layer is filled with 1 cm thick moist river sand. The twigs of the 10th layer were filled in blank. The 11th layer spreads evenly the granules of the honey ring fungus. The 12th layer covers 5 cm thick moist river sand. The 13th layer covers 5-6 cm of loose and wet leaves. Regulation temperature 20 °C -25 °C, adjust the space humidity 75% -85%. The leaves of the bed were kept moist and the hybrid hybrids of the 4-5 years matured.