First, Baoding should be performed before the insemination of the cow to ensure smooth insemination.

The second is that all devices used for insemination must be strictly disinfected before they can be used.

Third, the breeding staff must be skilled in thawing methods and insemination techniques, and the thawing of the semen should be promptly inseminated. After the insemination, make a record, which will help strengthen the management of the cows. Fourth, the thawing temperature of semen, the temperature of frozen semen thawing and the proper operation of the sperm directly affect the vitality of the sperm. At present, it is considered that the thawing effect is the best around 40°C. With the decrease of the thawing temperature, the sperm motility has a tendency of decreasing gradually.

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